25-08-2017, 09:32 PM
APPLICATION OF SOLID-STATE FERMENTATION FOR CELLULASE ENZYME PRODUCTION USING TRICHODERMA VIRIDE
30_V5_MACEDONIA_PIEB_Kiro Mojsov_d.pdf (Size: 72.37 KB / Downloads: 39)
Introduction
For solid-state fermentation (SSF) processes, different
agro-industrial wastes are used as solid substrates. Selection
of agro-industrial residues for utilization in SSF depends on
some physical parameters such as particle size, moisture
level, intra-particle spacing and nutrient composition within
the substrate. In recent years, some important agroindustrial
residues such as sugarcane bagasse, sugar beat
pulp/husk, orange bagasse, oil cakes, apple pomace, grape
juice, grape seed, coffee husk, wheat bran, cereals, straw,
leaves, corncobs etc. have been used as substrates for solidstate
fermentation.
Biomass production measurements
Biomass production was measured as dry weight (mg).
After filtering, the substrate and the retained cell mass was
dried at 70 oC in an oven until constant weight and then
measured. The content of protein in base was estimated
according to method of Keldhal [mg biomass = mg protein
X 2].
Results and discussion
Solid State Fermentation (SSF) is applied for the
processes in which insoluble materials in water are used for
the microbial growth (Moo-Young et al.,1983). Water is
essential for the microbial growth and in SSF and it is
present in thin layers and in occasions, absorbed inside the
substrates (Mudgett, 1986).
Conclusion
Agricultural waste in the form of cellulose which is the
most abundant renewable biomass in the biosphere has been
shown to be used in the production of valuable products by
microorganism. Wheat straw and alkali treatment wheat
straw are some of these agricultural wastes used in this
work as fermentation substrate which produced a large
amount of cellulase enzymes by Trichoderma viride. These
results highlight the industrial potentials of the substrates as
possible raw materials for cellulase enzyme production by
Trichoderma viride.