19-08-2014, 10:43 AM
Antibodies or immunoglobulins have emerged as an important class of biopharmaceuticals. Both Cohn fractionation used for purification of polyclonal IgG and Protein A affinity chromatography used for purification of monoclonal antibodies suffer from limitations. In the first part of work, an inexpensive yet effective pseudoaffinity adsorbent 'AbSep' was designed for purification of antibodies or IgG.
Antibodies or immunoglobulins have emerged as an important class of biopharmaceuticals. Both Cohn fractionation used for purification of polyclonal IgG and Protein A affinity chromatography used for purification of monoclonal antibodies suffer from limitations. In the first part of work, an inexpensive yet effective pseudoaffinity adsorbent 'AbSep' was designed for purification of antibodies or IgG. The effect of pH, conductivity and additive such as polyethylene glycol on binding capacity of AbSep for IgG was determined in order to optimize the binding and elution conditions for chromatographic process. AbSep was used to purify polyclonal IgG from human plasma, equine serum, goat serum and monoclonal antibody from cell culture supernatant with a high degree of recovery and purity. The second part of the work deals with the purification of ferulic acid from maize bran. Ferulic acid a potent antioxidant and a precursor for production of vanillin is present in maize bran at concentrations of (2-3%) dry weight. The principle of reversed phase chromatography was exploited for purification of ferulic acid. The overall purification process consisted of alkaline extraction of ferulic acid from maize bran and then purification of ferulic acid from extract by column chromatography using modified polystyrenic adsorbent.