27-11-2012, 01:03 PM
DESIGN AND PRODUCTION OF RECOMBINANT SUBUNIT VACCINES
Design Vaccine.ppt (Size: 2.74 MB / Downloads: 58)
Vaccination is the most cost-effective method of reducing animal suffering and economic losses due to infectious diseases in animals
The advancement of genomics, proteomics and biotechnology provide us the opportunity to develop safe and more effective vaccines
ACTIVE VACCINE
Stimulates Humoral Immune Response,Cellular Immune Response or Both, with the aim of protecting against or eliminating a pathogen
PASSIVE VACCINE
Preparation of Abs, Protect against a pathogen or disease and is administered before, at or around the time of known or potential exposure
RECOMBINANT SUBUNIT VACCINES
Identify and isolate a specific gene from
virulent bacteria or virus (gene that codes
immuno protective protein).
Gene is inserted into plasmid DNA and
ligated with ligase.
New (engineered) plasmid inserted into
another bacterium (transform).
Allowed to grow and actually produce
the antigenic protein.
The vaccine is comprised of purified
proteins recovered from the expression vector.
RECOMBINANT VECTOR VACCINES
Based on microorganisms such as viruses or bacteria that do not cause disease in target animals or humans.
The viruses or bacteria are used as vectors, or carriers, to deliver harmless genes into the cells of the body.
The body produces proteins from the genes and these proteins stimulate an immune response against the specific protein.
RECOMBINANT VIRAL-VECTOR VACCINE
Isolate an immunoprotective protein gene from a virulent virus.
Clone the gene to a vector of a non virulent virus.
These live vectored vaccines are being used to not only control infectious diseases of domestic animals, but of wildlife as well.
This approach has resulted in a dramatic reduction in transmission of RABIES from wildlife to domestic animals and humans. This would not have been possible by conventional methods.