25-08-2017, 09:32 PM
Optimization of inoculum size, temperature, carbon source, inorganic and organic nitrogen source and initial pH was done for production of SAM from spent brewerâââ‰â¢s yeast cells by one factor at -a- time.
Spent brewer's yeast, a by-product from the brewing industry, is being produced in large amount annually from beer manufacture due to increased volume of beer production. Yeast cells contain plenty of protein, lipid, RNA, vitamins and minerals. The brewer's yeast is an inexpensive nitrogen source generally recognized as safe (GRAS) and has good nutritional characteristics. It is sold primarily as an inexpensive animal feed and nutritional supplement after inactivation by heat because of high protein level (40 - 45 %). Use of spent yeast cells to produce value added products of commercial importance is an attractive option.
S-Adenosyl-l-methionine (SAM) is an important biochemical molecule participating in a number of biochemical reactions including transmethylation, trans-sulphuration and biosynthesis of aliphatic polyamines. Glutathione (GSH), the major non-protein thiol and a major natural endogenous hepatoprotective agent, is a low-molecular weight antioxidant that serves several biological functions. In this work, spent brewer’s yeast cells were used for fermentative production of S-Adenosyl-l-methionine and Glutathione.
Optimization of inoculum size, temperature, carbon source, inorganic and organic nitrogen source and initial pH was done for production of SAM from spent brewer’s yeast cells by one factor at -a- time.
Response surface methodology (RSM) was employed for optimization of major nutrients (glucose, l-methionine, ammonium sulphates and urea) for SAM production and results show that glucose, lmethionine and urea has significant effect on SAM production. Using RSM technique SAM production was increased by 1.81 fold. Media optimization for production of GSH from spent brewer’s yeast cells was done by method of one factor at -a –time and GSH production was increase by 1.97 fold.
An attempt was made to study the production of SAM and GSH in 1-liter bioreactor. SAM production was found to be 32.43 mg/DCW.. Purification of SAM from broth will be done by ion exchange chromatography.