19-08-2014, 11:55 AM
Phytase is a myo-inositolhexakisphosphatase occurs in seeds and cereals like mung bean, soybean, wheat and barley. It degrades phytic acid (myo-inositolhexakisphosphate) into free phosphate, minerals and myo-inositol or its derivatives. Phytase enzyme activities were observed in germinated mung bean, soybean and wheat. Out of these wheat gives the highest activity.
Phytase is a myo-inositolhexakisphosphatase occurs in seeds and cereals like mung bean, soybean, wheat and barley. It degrades phytic acid (myo-inositolhexakisphosphate) into free phosphate, minerals and myo-inositol or its derivatives. Phytase enzyme activities were observed in germinated mung bean, soybean and wheat. Out of these wheat gives the highest activity. Phytase enzyme was extracted from wheat in Sodium acetate buffer. Effect of germination time, cell wall lytic enzymes, protease inhibitor and surfactant on the extraction of enzyme was studied. Phytase activity was highest after two days of germination. A cell wall lytic enzyme such as cellulase and pectinase increases the extraction of intracellular phytase enzyme by breaking cell wall. Triton X-100, which is non-ionic surfactant, degraded the cell membrane and helps in extraction. Protease inhibitor phenylmethylsulfonyl fluoride decreases the possibility of degradation of phytase by blocking protease enzyme. Purification of phytase is in progress and efforts are on to characterize the phytase enzyme from wheat