19-08-2014, 11:58 AM
Due to the high region-, stereo- and anomeric selectivity of b-glucosidases enzymatic approach for the synthesis of alkyl and aryl glucosides has attracted considerable interest for several years in the context of green chemistry and sustainable development.
b-glucosidase (-D-glucoside glucohydrolase; EC 3.2.1.21) is the enzyme of commercial interest in pharmaceutical, food, starch, and cosmetic industries -Glucosidase belongs to large class of hydrolases enzyme and catalyzes the cleavage of b- glucosidic ether bond of alkyl and aryl glucosides. b-glucosidase can also synthesize alkyl and aryl glucosides through transglycosylation and reverse hydrolysis reactions.
Due to the high region-, stereo- and anomeric selectivity of b-glucosidases enzymatic approach for the synthesis of alkyl and aryl glucosides has attracted considerable interest for several years in the context of green chemistry and sustainable development.
The present work involves the purification of b-glucosidase enzyme from almonds (Prunus amygdalus), fermentative production of b-glucosidase from Trichoderma reesei NCIM 1052 and Alcaligenes faecalis NCIM 2444 and purification to significant fold purity by different purification and chromatographic steps. We have also tried immobilization of b-glucosidase from almonds and Trichoderma reesei by techniques such as entrapment (Calcium alginate), adsorption (Amberlite XAD-4), cross-linking (Chitosanglutaraldehyde).
The work also involves enzymatic approach to synthesize glucosides in anomerically pure form by the reverse hydrolysis and transglycosylation reactions by immobilized enzyme.