21-12-2012, 11:43 AM
HUMAN ADENOVIRUS
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PLANT CELL LINE
Very first culture explant the cell it is grow with media to produce colony it is called primary culture. after subculturing it is called cell line.
APPLICATIONS OF CELL LINE
Cell line are uniform crop of cell maintain time to time to carry out research and testing.(Physiological , Biochemical & Pharmaceutical)
The uniformity of cells can ensure reproducibility.
These are true hybrid clones escaping natural process of evolution & variation therefore, it is a good material for experimental study.
The consistency of the randomness of mutagens or oncogenes could be verified using cell line.
Some times clones produce by cell line to provide commercially important secondary metabolites. For e.g. wine producing yeast cells, antibiotic producing bacteria, herbal medicine production through plant cell lines.
Cell lines are also used for invitro drug testing. e.g. antiviral , anti bacterial , antitumor , antimalarial etc..
PLANT CELL LINES MAINTENANCE
Clonal propagation/ Micro propagation
- It has been demonstrated that a variety of plant species can be conveniently propagation through the technique of cell, tissue or organ culture this is called clonal propagation.
Why we are using clonal propagation?
Because this method has major benefits.
(1)For the rapid multiplication of superior clones and maintains of uniformity.
(2)It also useful in multiplication of disease free plant.
(3)Multiplication of sexually derived sterile hybrids.