19-08-2014, 11:54 AM
Research work was carried out to isolate ambrettolide from Ambrette seed oil and its characterization.
Research work was carried out to isolate ambrettolide from Ambrette seed oil and its characterization.
Ambrette seeds were powdered and extracted with methanol in extractor with heating and continuous stirring. Extracted oil was saponified with aqueous KOH to remove the fatty acids present in the oil.
Saponified matter was fractioned with hexane to remove impurities. This hexane layer was fractioned with methanol. Repeated fractionation resulted in pure ambrettolide. Isolated compounds were characterized by GC and GC-MS.