21-03-2014, 02:16 PM
MONOCLONAL ANTIBODIES
MONOCLONAL ANTIBODIES.ppt (Size: 197.5 KB / Downloads: 97)
Preparing monoclonal antibodies
Inject pathogen against which antibody is to be raised into a mouse ©
Mouse immune system stimulated to produce antibodies against antigens on the pathogen’s membrane (there will be several different antibodies at this stage = polyclonal) (A)
Antiserum (blood serum rich in antibodies) extracted from mouse.(D)
Mouse B cells fused with tumor B cells to create a immortal cell lines for producing antibodies (called an ‘hybridoma’ or ‘myeloma’). (G)
Hybridoma cells allowed to multiply in-vitro on nutrient medium. (I)
Each hybridoma cell line produces the antibody from the B cell it fused with (B)
Therefore some colonies will produce the desired antibody. (H)
Hybridoma colonies are screened to identify those which produce the desired antibody. (E)
Clone selected and cultured to produce the “Monoclonal Antibody” (F)
[b]Text pages 109-111[/b]
List the four main applications of monoclonal antibodies
Produce a table highlighting the benefits and disadvantages of this technology. Include as many ‘ethical’ issues as possible.
Answer exam style Q 3 on page 117.