03-10-2012, 12:36 PM
Analytical Method Development and Validation
Analytical Method.pdf (Size: 550.12 KB / Downloads: 108)
CBDA Grant Study Goal:
Develop and validate sample extraction and
analysis procedures that give accurate and
reproducible results for environmentally relevant
concentrations of pyrethroid insecticides (home
and commercial use)
Methods
Compatible with GC and HPLC analyses
Applicable to water, colloids, sediment and biota
Applicable at environmentally relevant concentrations
Amenable to automation for high sample throughput
Cost effective
Development of Analytical Methods
Optimization (sensitivity and selectivity) of instrument
methods used for analysis and confirmation (GCECD,
GC-MS, LC-MS, GC-MS/MS and LC-MS/MS)
Isomer, enantiomer and stereoisomer identification
and stability (deltamethrin, tralomethrin)
Evaluation of water extraction methods (liq-liq, SPE,
SPME)
Evaluation of sediment and tissue extraction and
matrix separation methods (PFE, GPC, Florisil)
SUMMARY
Methods developed and validated for water,
sediment and tissue
Method of quantitation - total vs. isomers
Method extends to other pyrethroids –
allethrin, flucythrinate, phenothrin, prallethrin,
tetramethin and tralomethrin
GC/MS/MS (TSQ): Very sensitive – at least 2 – 10
times lower than GC/ECD; best tool to obtain
lower reporting limit by reducing background
interference