25-01-2013, 03:30 PM
Optical Biosensors and Applications
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What is an Optical Biosensor
BiosensorBiosensor
Measures changes in optical properties of substancessubstances
– Absorbance in chemical reaction
– Fluorescence
– Reflectance
– Refractive index
– Phase shift
– Light Energy (wavelength)
Reaction will cause LuminescenceReaction Luminescence
Types of Optical Biosensors
Four main types:
1. Fiber Optics
a. Indicator mediated
b. Immunoassay
2. Surface Plasmon Resonance (SPR)
-Immunoassay
3. Absorbance
4. Luminescence
Light Propagation
Through Optical Fibers
Refractive index of core is greater than claddingRefractive cladding
Light propagates through the coreLight core
Total internal reflectionTotal reflection
Light of different wavelengths will not interfereLight interfere