14-01-2013, 04:46 PM
Plant Tissue Culture
1Plant Tissue.ppt (Size: 91 KB / Downloads: 46)
What is Tissue Culture?
Asexual Propagation
aka Micropropagation
Defined as:
Production of new plantlets from tissue of an actively growing plant
Why use tissue culture?
Species with any of the following:
Regeneration problems
Poor germination
Desirable traits
Disease-resistance
High productivity
Gender of commercial importance
Male- Asparagus
Female- Papaya
What is required?
Very sanitary conditions
Usually a lab
Sterile aseptic culture or MEDIA containing:
Nutrients
Sugars
Vitamins
Hormones
Test tube
Plant tissue:
shoot tip, leaf section, meristem, node, etc.
The Procedure
Prepare the media
Sterilize everything including:
Media
Tools
Supplies
Equipment
Work area
Place media in test tube
Remove tissue from plant
Sanitize tissue sample
Place tissue sample on media in test tube
Cap or seal test tube
Place in warm, well-lighted area
Plantlets should begin to form in 4-6 weeks