19-12-2012, 04:05 PM
Plant Tissue Culture
Plant Tissue Culture.pptx (Size: 1.05 MB / Downloads: 67)
Introduction to Tissue Culture
Tissue Culture (also known as Micropropagation or In vitro culture) is:
The growing of plant cells, tissues, organs, seeds or other plant parts in a sterile environment on a nutrient medium.
Picture Dictation Exercise
Take some meristematic cells from a plant.
These cells are called an explant
Place the explant on a sterile nutrient rich agar
The explant grows into a ball of cells
This ball of cells is called a callus
The callus then develops roots, stem and leaves
This is called a plantlet
Transplant the plantlet into a traditional growing media
What is Callus development
A callus is a blob of tissue – (mostly undifferentiated cells)
A callus is naturally developed on a plant as a result of a wound
This callus can be left to develop or can be further divided