22-08-2013, 03:18 PM
Quantitative Histochemical and Immunohistochemical Studies on the effects of selenium and zinc on lead-induced testicular damage in Sprague-Dawley rats
Quantitative Histochemical.ppt (Size: 297.5 KB / Downloads: 14)
Introduction
Lead is an abundant heavy toxic metal which is known to induce a broad range of physiological and behavioral dysfunction in humans1.
Associated with several clinical symptoms and a limited understanding of the molecular mechanisms underlying the toxicity.
Toxic metals have in common the ability to cause oxidative damage and increase the production of free radicals while decreasing antioxidant reserves5.
Main sources absorption is inhalation and ingestion. (Merrill, Morton and Soileau (2007)
In August 2009, parents rioted in China aftermath of poisoning in about 2000 children. Watts (2009)
Zamfara’s case claimed 153 lives according to Government’s figures Nigeriansfornigeria.org
Selenium
Selenium is an important element responsible for male fertility and testosterone biosynthesis6,
component of deiiodonidase enzyme, which transform T4 (thyroxine) into T3 (Tri iodothyronine)8 and plays a vital role in oxidative stress control as a component of the glutathione peroxidase system
It’s deficiency has also been associated with nutritional muscular dystrophy9.
Zinc
Zinc is a trace element essential for living organism. More than 300 enzymes require zinc as a co-factor for their activity.
Plays an important role in DNA replication, transcription and protein synthesis and therefore influencing cell division and differentiation13
Immuno histochemistry
Immunohistochemistry(IHC) refers to the process of detecting antigens (e.g., proteins) in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues. Ramos-Vara, JA (2005).
The aim and objectives.
The aim
The overall aim of this study is to determine the effect of co-administration of zinc and selenium supplementation on lead-induced testicular damage
Materials and Methods
Experiments were performed on 140 Sprague-Dawley rats (200g), obtained from the animal house department of Anatomy, College of Medicine University of Lagos. Nigeria. The animals were housed in wire meshed cages and maintained in a well ventilated room at 25±2 ˚C on a 12hr light and dark cycle with free access to rat chow (Bendel Feeds and Floor Mills Ltd, Benin City, Nigeria) and drinking water.