25-08-2017, 09:32 PM
SEMESTER EXAMINATIONS FOR GENETIC ENGINEERING
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Answer any five questions
All questions carry equal marks
1. What is Sanger dideoxy sequencing and how it is different from fluorescent sequencing.
2. How do you obtain an expression of eukaryotic gene in bacteria? Discuss about the procedure used?
3. Write brief notes on
a) Nothern blotting.
b) Plasmids.
4.a) What is the uniqueness in the sigma switch of Bacillus subtilus? Explain the role and mechanism of action.
b) Discuss the importance of promoter and enhancer elements.
5. Write short notes on
a) Mechanism of Transposition.
b) F+ - F- mechanism in bacteria.
c) Gene silencing.
6. Describe the Tryptophan operon structure and function. Explain the mechanism in its regulation.
7. Explain the following
a) Relaxed Plasmids.
b) Multiple cloning sites.
c) Retro transposons.
8. Give a detailed account on the construction of genomic libraries.