05-03-2013, 01:02 PM
Synthesis of NPs using Proteins and templates like DNA.
Synthesis of NPs.pptx (Size: 211.28 KB / Downloads: 31)
Ferritin
Aboundent in animals
Ferritin - Colloidal Protien of nanosize
Monomer subunit
Protein structure
Stores Fe in metabolic process
Form 3D hierarchial str; 24 prt subunits (central activity-6nm); Diameter of polypeptide shell is 12nm
Ferritin can accomidate 4500 Fe atoms(They are in Fe3+ state)
The protein subunits are composed of light as well as heavy chains having dinuclear ferroxide centres( these centres are catalysts for invtro oxidation of Fe2+
Apoferritin Synthesis of CdS NPs
Process of CdS formation is stepwise
Ferritin + Na. acetate buff Dialysis bag
Bag Soln of Na. acetate + Thioglycolic acid (N2 gas flow for 2Hrs)
Further dialysis of Apoferritin soln done against saline 20Hrs
Mix Apoferritin(NaCl+ TES)
Addition of Cd. Acetate
Na2 S is added twice in 1 Hr interval
Cd loading 55 atoms per Apoferritin colloidal taking place in each step
Manganes, uranyl oxide, Cobalt, Pt etc being synthesised inside ferritin
synthesis using DNA
Nucleosides and nucleotides( Nucleosides consist of a nucleobase (A, T, G or C) and a sugar (RNA, DNA) ring. NTPs are triphosphorylated versions of nucleosides)
Nucleic acid sequences are able to recognize or catalyse the formation of NP s with specific morphologies. The systematic evolution of ligands by exponential enrichment processapplied to the selection of RNA sequences catalysing the formation of Pd NP s. This process starts from a library of DNA sequences containing a stretch of randomized nucleotides in all possible permutations. Transcription of the DNA library