06-07-2012, 04:42 PM
BIOTECHNOLOGY IN FORENSIC SCIENCE
biotechnology in forensic study.ppt (Size: 904 KB / Downloads: 44)
THE BEGINNING OF THE REVOLUTION
DNA analysis was first described in 1985 by Dr. Alec Jeffreys. The technique developed called restriction fragment length polymorphism(RFLP).
PCR-Based Tests
Reverse dot blot technique’
Minisatellite variant repeat (MVR) mapping
Advantage
Availability of commercial
Rapid results
High degree of sensitivity
ADVANTAGES
It is present in high copy number.
Provide better results when nuclear DNA is scanty, e.g., analysis of hair shafts, teeth,
mtDNA is transmitted exclusively maternally to the offspring without undergoing recombination.
DISADVANTAGE
Heteroplasmy (same individual harboring more than one mtDNA sequence).