26-05-2012, 12:51 PM
Extraction, Identification and Antibacterial activity of Cyperus oil from Iraqi C. rotundus
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Introduction
C. rotundus has many different
uses were based on the different parts
of the plant. The medical use of
cyperus has been used in medicine
for thousands of years. The parts of
the cyperus used are its leaves, seeds
and oil. The fresh leaves and ripe
seeds have quite different aromas and
uses. Both the leaves and seeds are
rich in volatile oils that act mainly on
the digestive system, stimulating the
appetite and relieving irritation and
as an expectorant. The oil is
fungicidal and bactericidal. The,
leaves were widely used to flavor
food, especially in the Middle East,
and Southeast Asia. The seeds are
also an ingredient of curries and
pickling spices, dishes a la grecque,
and bakery products.
Identification of cyperus oil composition (8):
Examine by thin-layer
chromatography using a silica gel as
the coating substance.
Test solution. 0.50 mL of cyperus oil
was shaken with 5.0 ml of hexane for
2 min to 3 min and filter over 2 g of
anhydrous sodium sulphate
Reference solution. 15 μl of each of
the following standard solutions
cyperol, cyperene, cyperone,
rotundine, caryophyllene and 25 μl
of olive oil were dissolved in 5.0 ml
of hexane immediately before use.
Extraction of Cyperus oil:
C. rotundus ripe dried fruits were
broken into small pieces under sterile
conditions; 20 g of powdered fruits
were dissolved with 150 mL of
ethanol, ethyl acetate, methanol and
chloroform solvents then extracted
by using steam distillation apparatus
for 30 minutes. The determination of
Cyperus oil was carried out by the
steam distillation apparatus. The
distillate was collected in a graduated
separatory funnel. The water-free
mixture of volatile oil was recovered
as follows:
Results and Discussion
In this study, extraction
procedure was conducted by
steam distillation, the best method
for extraction of the essential oil
containing hydroxyl groups or
other polar groups via formation
oxonium extraction system,
Optimum extractant was
estimated depending on the
quantity of cyperol that extracted
by the following solvents;
ethanol, ethyl acetate, methanol
and chloroform in the study we
conclude that Methanol was the
best extractant, Table (1).