A fungal cell is designed with a circular groove and its dispersion diagram is compared to the normal fungus cell. Next, a patch antenna placed on a substrate constructed from the mushroom cell with circular groove is designed and compared to a patch antenna with the same dimensions placed on the FR4 substrate. A patch antenna filled with mushroom cell with circular grooves, then a patch antenna filled with the circular mushroom cell and placed on a metamaterial substrate constructed from the mushroom cell with circular groove is designed to operate The results show that a metamaterial patch antenna with a metamaterial substrate is a good candidate for the Multiple Input Multiple Output (MIMO) communication system, ). However, an additional improvement is needed for its gain and efficiency.