The worldwide food protein deficiency is becoming alarming day to day and with the fast growing population of world,
pressure is exerted on the feed industry to produce enough animal feed to meet the region’s nutritional requirements.
Single-Cell Protein (SCP) represents microbial cells (primary) grown in mass culture and harvested for use as protein
sources in foods or animal feeds. In the present study, pineapple waste was used as sole carbon source in five
concentrations for preparation of fermentation media on which two strains of yeasts, Saccharomyces cerevisiae and
Candida tropicalis were grown. The increased concentration of pineapple hydrolysate enhanced the biomass yield and
the protein formation within the yeast cells. Lower carbon utilization by the two yeast strains occurred in the wastecontaining
media, as compared to control, increasing the economic value of the waste obtained after 7-day fermentation.
The present finding helps in SCP production from cheap, inexpensive agro waste material.
Holocellulose structures from agro-industrial residues rely on main and side chain attacking enzymes with different specificities for complete hydrolysis. Combinations of crude enzymatic extracts from different fungal species, including Aspergillus terreus, Aspergillus oryzae, Aspergillus niger and Trichoderma longibrachiatum, were applied to sugar cane bagasse, banana stem and dirty cotton residue to investigate the hydrolysis of holocellulose structures. A. terreus and A. oryzae were the best producers of FPase and xylanase activities. A combination of A. terreus and A. oryzae extracts in a 50% proportion provided optimal hydrolysis of dirty cotton residue and banana stem. For the hydrolysis of sugar cane bagasse, the best results were obtained with samples only containing A. terreus crude extract.