04-03-2011, 10:38 AM
PRESENTED By:
Mohammad Hossein Banitaba
[attachment=9507]
History of surface plasmon resonance
• Since its first observation by Wood in 1902 ,the physical phenomenon of surface plasmon resonance (SPR) has found its way into practical applications in sensitive detectors, capable of detecting sub-monomolecular coverage.
• Wood observed a pattern of ‘‘anomalous’’ dark and light bands in the reflected light, when he shone polarized light on a mirror with a diffraction grating on its surface.
• a complete explanation of the phenomenon was not possible until 1968, when Otto and in the same year Kretschmann and Raether reported the excitation of surface plasmons.
• Application of SPR-based sensors to biomolecular interaction monitoring was first demonstrated in 1983 by Liedberg et al.
What is Surface Plasmon Resonance?
• The SPR angle is dependent on the optical characteristics of the system, e.g. on the refractive indices of the media at both sides of the metal.
• the refractive index in the immediate vicinity of the metal surface will change when accumulated mass (e.g. proteins) adsorb on it. Hence the SPR conditions are changing and the shift of the SPR angle is suited to provide information on the kinetics adsorption on the surface.
• Sensogram
• SPR vs. QCM
• Instrumentation
SPR instruments comprise three essential units integrated in one system.
• The Steps of an SPR Assay
• SPR Liquid Handling Systems
Detection of Wild-Type and Mutant p53 Proteins in Cancer Cell Lysates
• Quantitative application
Protein/Drug Interaction: Ferulic Acid and Bovine Serum Albumin
• Small molecules
• In many biosensors, silane coupling chemistry on oxide substrates (glass, silicon, indium tin oxide) is used for immobilization of biomolecules.
Electrochemical SPR
Gas Phase SPR
SPR as a Chromatographic Detector: Separation and Label-Free Detection of Biomolecules
Conclusion
• Surface Plasmon Resonance (SPR) is a powerful technique to measure biomolecular interactions in real-time in a label free environment. While one of the interactants is immobilized to the sensor surface, the other are free in solution and passed over the surface. In addition, the binding of two interactants can be exploited to quantify the concentration of one of the interactants.
Mohammad Hossein Banitaba
[attachment=9507]
History of surface plasmon resonance
• Since its first observation by Wood in 1902 ,the physical phenomenon of surface plasmon resonance (SPR) has found its way into practical applications in sensitive detectors, capable of detecting sub-monomolecular coverage.
• Wood observed a pattern of ‘‘anomalous’’ dark and light bands in the reflected light, when he shone polarized light on a mirror with a diffraction grating on its surface.
• a complete explanation of the phenomenon was not possible until 1968, when Otto and in the same year Kretschmann and Raether reported the excitation of surface plasmons.
• Application of SPR-based sensors to biomolecular interaction monitoring was first demonstrated in 1983 by Liedberg et al.
What is Surface Plasmon Resonance?
• The SPR angle is dependent on the optical characteristics of the system, e.g. on the refractive indices of the media at both sides of the metal.
• the refractive index in the immediate vicinity of the metal surface will change when accumulated mass (e.g. proteins) adsorb on it. Hence the SPR conditions are changing and the shift of the SPR angle is suited to provide information on the kinetics adsorption on the surface.
• Sensogram
• SPR vs. QCM
• Instrumentation
SPR instruments comprise three essential units integrated in one system.
• The Steps of an SPR Assay
• SPR Liquid Handling Systems
Detection of Wild-Type and Mutant p53 Proteins in Cancer Cell Lysates
• Quantitative application
Protein/Drug Interaction: Ferulic Acid and Bovine Serum Albumin
• Small molecules
• In many biosensors, silane coupling chemistry on oxide substrates (glass, silicon, indium tin oxide) is used for immobilization of biomolecules.
Electrochemical SPR
Gas Phase SPR
SPR as a Chromatographic Detector: Separation and Label-Free Detection of Biomolecules
Conclusion
• Surface Plasmon Resonance (SPR) is a powerful technique to measure biomolecular interactions in real-time in a label free environment. While one of the interactants is immobilized to the sensor surface, the other are free in solution and passed over the surface. In addition, the binding of two interactants can be exploited to quantify the concentration of one of the interactants.