03-08-2012, 02:07 PM
GEL ELECTROPHOROSIS
[attachment=30041]
Electrophoresis
Electrophoresis is the movement of charged particles through an electrolyte when subjected to an electric Field
Cations move towards cathode
Anions move towards anode
By this technique solutes are separated by their differentrates of travel through an electric field.
Commonly used in biological analysis, particularly in the separations of proteins, peptides and nucleic acids
Factors affecting electrophoresis
The rate of migration of a solute in an electric field depends on the following factors-
Net charge on the particle
Mass and shape of the particles
pH of the medium
Strength of electric field
Properties of supporting medium
Temperature
What is Gel Electrophoresis
Gel Electrophoresis is a process that can be used to separate DNA Fragments
It uses an electric current to separate the pieces of DNA that are run through the gel
The gel used has agarose, a highly purified sea weed, in it.
It can separate DNA molecules from hundreds of nucleotides to hundreds of thousands of them
Running the gel
Since DNA has a negative charge, It will move toward the positive end of the gel tank when electricity is run through the solution.
The way in which it moves is called reptation, because it moves like a snake through the porous gel.
Smaller fragments move faster and further thanlarger fragments, allowing for separation
How it changed Science?
Gel Electrophoresis affected science in a big way.
Scientists now had a relatively easy and definitive way to test and compare DNA samples.
It lead the way for more specialized and complex DNA testing
Gel Electrophoresis has become an absolute necessity in the field of Forensics
Is usually only used for the good of society, such as crime fighting, suspect identification, paternity testing, etc.
[attachment=30041]
Electrophoresis
Electrophoresis is the movement of charged particles through an electrolyte when subjected to an electric Field
Cations move towards cathode
Anions move towards anode
By this technique solutes are separated by their differentrates of travel through an electric field.
Commonly used in biological analysis, particularly in the separations of proteins, peptides and nucleic acids
Factors affecting electrophoresis
The rate of migration of a solute in an electric field depends on the following factors-
Net charge on the particle
Mass and shape of the particles
pH of the medium
Strength of electric field
Properties of supporting medium
Temperature
What is Gel Electrophoresis
Gel Electrophoresis is a process that can be used to separate DNA Fragments
It uses an electric current to separate the pieces of DNA that are run through the gel
The gel used has agarose, a highly purified sea weed, in it.
It can separate DNA molecules from hundreds of nucleotides to hundreds of thousands of them
Running the gel
Since DNA has a negative charge, It will move toward the positive end of the gel tank when electricity is run through the solution.
The way in which it moves is called reptation, because it moves like a snake through the porous gel.
Smaller fragments move faster and further thanlarger fragments, allowing for separation
How it changed Science?
Gel Electrophoresis affected science in a big way.
Scientists now had a relatively easy and definitive way to test and compare DNA samples.
It lead the way for more specialized and complex DNA testing
Gel Electrophoresis has become an absolute necessity in the field of Forensics
Is usually only used for the good of society, such as crime fighting, suspect identification, paternity testing, etc.