02-09-2017, 02:32 PM
In the present study, partially deproteinated paneer serum (0.1-0.2% w / v residual protein after deprotection), which resembles cottage cheese whey, originated from buffalo milk ( containing 3.85-4.95% w / v lactose) for possible rapid recovery of lactose using 'anti-solvent' (ethanol). Optimization of various process parameters, such as effective ethanol concentration (65-85% v / v), pH, temperature, seeding, etc. were carried out. In the optimization of the process, recovery of lactose of greater than 90% and purity of 97-99% were obtained from paneer serum in 1 h stirring time, at an effective ethanol concentration of 85% v / v. The values of "roundness" (shape factor) and "elongation" (aspect ratio) of the lactose crystals recovered in the present study were found to be comparable to those of the commercial analytical grade lactose sample. Crystallization kinetics has also been studied for lactose recovery using a recovery process based on anti-solvent ethanol.