12-04-2013, 03:06 PM
M.TECH I SEMESTER REGULAR EXAMINATIONS MARCH 2010 BIO CHEMICAL ENGINEERING
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1. Derive the Michealis-Menten equation by using
a) The rapid equilibrium assumption and
b) quasi-steady state assumption
for simple enzyme kinetics that involves a reversible step for enzyme-substrate
complex and a dissociation step of the ES complex.
2. Write in detail about active and passive immobilization of cells.
3. Aerobic degradation of benzoic acid by a mixed culture of microorganisms can
be represented by the following reaction.
C6H5COOH + a O2 + b NH3 c C5H7NO2 + d H2O + e CO2
(Substrate) (Bacteria)
a) Determine a, b, c, d, and e if RQ = 0.9
b) Determine the yield coefficients, YX/S and YX/O2
c) Determine degree of reduction for the substrate and bacteria.
4. Give a brief account of packed bed bioreactors and trickle bed bioreactors.
5. a) Discuss in detail about thermal death kinetics.
b) Explain the process of sterilization of air.
6. Give a detailed account of alternate bioreactor configurations.
7. a) Define simple and complex media. Give an example for each.
b) Discuss about medium requirements and medium formulation.
8. With respect to mass transfer into solid particles, write about
a) External mass transfer resistance
b) Intra-particle diffusion and reaction