06-08-2013, 04:48 PM
Expression profiling of pathogenicity genes of Magnaporthe grisea to understand rice blast disease spectrum
ABSTRACT
Blast caused by Magnaporthe grisea is one of the most devastating diseases of rice in the world. To understand the molecular mechanism of rice blast disease, an experiment was conducted under glass house to test the hypothesis that magnitude and time of expression of genes associated with fungus development and colonization determine the disease spectrum in resistance and susceptible genotypes. Thirteen different primer sets (CHM1, MPLC1, LpMOD, ABC1, CAMGEN, CALMOD, OSM1, TRE1, UEP1, MPS1, MAC1, TPAGEN and MAGNA) encoding virulence genes were designed and validated in silico using various bioinformatics tools. Real time PCR and plant bioassay tests were used to monitor the expression of genes at different time points after pathogen challenged inoculation in resistant and susceptible genotypes. Data analysis indicates that all the fifteen genes were involved in the disease development under artificial epiphytotic conditions, although level of gene expression varied during compatible and incompatible interactions. These findings revealed that there might be a great possibility of regulation of rice blast disease spectrum by these genes under natural conditions.