07-08-2013, 01:08 PM
Isolation and molecular identification of superficial skin mycosis
ABSTRACT
Fungal pathogens have been reported to cause infections in human beings suffering from various diseases such as Cancer, Diabetes, AIDS etc. These fungal infections are mostly caused by saprophytes that are generally harmless in the environment but become opportunistic when the immune system of patients become compromised or suppressed. In the present investigation, we have taken the patients suffering from acute skin infection in various body parts like skin and nails. Case profile of the patient was recorded with various parameters such as age, occupation and medical case history etc. taken as consideration. The main cause of fungal infection is occupational hygiene, the infection were more prevalent among farmers than other professions. Farm work is generally associated with developing higher risk of superficial skin mycosis, because they mostly deal with cattle’s, pets, and garden soil etc. The patient suffering from the fungal infection shows symptoms like unpleasant skin changes, secondary allergization, inflamed skin, formation of skin pustules etc. promotes other microbe to invade the body through this infection. Isolation of fungal samples were carried out and about 38 total cultures were isolated, purified and maintained with a suitable media i.e. SDA. The molecular studies were further planned out with selected cultures and processed for ITS based identification. Fungal samples are typically identified to species level through DNA sequencing of the nuclear internal transcribed spacer (ITS) region for use in BLAST-based similarity.