22-04-2014, 04:15 PM
Pure Culture Removal of Textile Dye from The Wastewater: Comparing E.coli and B. subtillis
Abstract
In the current era dyes play a vital role in industries. Extensive use of dyes has lead to enormous risks to all species
including human beings. Biological treatment of these dyes is one of the most versatile options we have. This work is based on the
utilization of microorganisms endowed with degrading abilities for the detoxification of harmful dye. The pure culture namely E.
coli and bacillus subtilis are compared for their ability to degrade the dye. Experiments were conducted at 2 glucose
concentrations and several dye concentration (50, 100, 150 and 200 ppm). Results show that dye degradation was most effective at
2 g/L for Bacillus subtillis, whereas it was observed to be 1 g/L for E.coli. Although glucose is universally biodegradable
compound, there is a complex interaction between different primary substrates and intermediate products resulting in contradicting
effects. The results were also compared with immobilized cells of E. coli and Bacillus subtillis. The immobilization resulted in
higher degradation. Moreover there was variation in effect of glucose concentration on the degradation. In both cases, higher
degradation was achieved at 1 g/L glucose concentration.