11-06-2013, 02:14 PM
SEMESTER EXAMINATIONS FOR DOWNSTREAM PROCESSING
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Answer any five questions
All questions carry equal marks
1.a) Write in detail about the selecting of various unit operations in relation to their separation factor and the molecular size of the material to be recovered in DSP.
b) Write short notes on classification of cell disruption methods and explain them.
2.a) Explain briefly the classification of precipitation processes.
b) What are the six major requirements of an effective adsorbent?
3.a) Discuss the application of batch settling tests in design of a Continuous Thickener.
b) Compare single effect evaporators with multiple effect evaporators and explain the different methods of feeding a multiple effect evaporator.
4. Filter aid and filter medium are used to isolate an antibiotic from a fermentation broth. For the collection of filtrate, volume of 0.1 litre took around 25sec. Data during this process is obtained by plotting (At/V) which is linear. The intercept shows that the medium has no significant resistant. The total area of the filter medium is 0.29 ft2. The filtrate has a viscosity of 1.129 cP and the pressure drop is 24 inch of mercury. The feed contains 0.018 kg dry cake per litre. Calculate the specific cake resistance.
5.a) Describe the construction details of the industrial freeze dryer.
b) Explain the different stages of crystallization.
6.a) Determine the flux assuming that there is no effect of concentration polarization during ultrafiltration of fermentation broth containing 0.98 wt% protein at a pressure difference of 5 kf/atm psi and the membrane permeability is 1.4 × 10-2 kg/s m2 atm.
b) Explain the principle, theory and application of dialysis.
7.a) Describe the theory and practice of reverse phase chromatography.
b) Write a note on advantages and application of gel permeation chromatography.
8.a) One hundred kilograms of a 50% solution of C in A (carrier) is equilibrated with 70 kg of solvent B containing 2% of C. At equilibrium, the raffinate phase has a mass of 80 kg and has 52% A and 8% B in it. What is the selectivity?
b) Explain briefly how to estimate maximum and minimum solvent flow rate in liquid–liquid extraction.
c) Write the classification of liquid –liquid extraction equipments.