02-09-2017, 04:42 PM
we investigated the characterization of an isolation of Aspergillus niger-producing amylase, optimizing the composition of the medium, cultural conditions for the production of amylase enzymes, extraction and partial purification of the extracellular enzyme from a potential isolation using free and immobilized. A. niger alpha-amylase had the optimum pH between 4 and 6 and the optimum temperature varied between 30 and 40 ° C, however the optimum pH, temperature and incubation period for the production of enzymes were 5.0, 35 ° C and 5 ° day immobilized cells and 5.0, 30 ° C and 5 th day for free cells, respectively. Of the carbon sources, starch was recorded as the best source of carbon for the production of enzymes. 0.03% peptone was the ideal source of nitrogen. However, the surfactants Tween-80, Triton X-100 and 0.02% sodium dodecyl sulfate. 0.002% and concentration of 0.0002% were more effective for the improvement of α-amylase production. Finally, the enzyme was characterized by chromatography and confirmed as alpha-amylase.
The α-amylase enzymes are all α-1,4-glucan-4-glucanohydrolases (EC 3.2.1.1) which are important enzymes used in the starch processing industries for the hydrolysis of polysaccharides such as starch into simple sugar constituents according to as reported by Akpan et al. (1999a, 1999b); Fogarty et al. (1980); Haq et al. (2002). Starch-degrading enzymes such as amylase have received great attention because of their perceived technological importance and economic benefits. This enzyme is also used for the commercial production of glucose. In storage tissues such as seeds, the glucose polysaccharide starch is hydrolyzed for use by growing seedlings to meet their energy requirement. Nowadays, the new potential of the use of microorganisms as biotechnological sources of relevant industrial enzymes has stimulated renewed interest in the exploration of the extracellular enzymatic activity in several microorganisms.
The α-amylase enzymes are all α-1,4-glucan-4-glucanohydrolases (EC 3.2.1.1) which are important enzymes used in the starch processing industries for the hydrolysis of polysaccharides such as starch into simple sugar constituents according to as reported by Akpan et al. (1999a, 1999b); Fogarty et al. (1980); Haq et al. (2002). Starch-degrading enzymes such as amylase have received great attention because of their perceived technological importance and economic benefits. This enzyme is also used for the commercial production of glucose. In storage tissues such as seeds, the glucose polysaccharide starch is hydrolyzed for use by growing seedlings to meet their energy requirement. Nowadays, the new potential of the use of microorganisms as biotechnological sources of relevant industrial enzymes has stimulated renewed interest in the exploration of the extracellular enzymatic activity in several microorganisms.