27-12-2012, 05:21 PM
Cloning strategies
Cloning strategies.ppt (Size: 1.89 MB / Downloads: 30)
TA Cloning
exploits the terminal transferase activity of some DNA polymerases.
This enzyme adds a single, 3'-A overhang to each end of the PCR product.
The PCR products with dA overhang, are mixed with this vector with single 3'-T overhangs in high proportion.
The complementary overhangs of "T" vector and PCR product will be ligated under the action of T4 DNA ligase.